Coding

Part:BBa_K3617002:Design

Designed by: David Nørgaard Essenbæk   Group: iGEM20_UCopenhagen   (2020-10-22)


Interleukin-10 receptor type 1 - C-ubiquitin-LexA-VP16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 628
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1269
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose to leave in both extracellular domains, since both looked to be part of interleukin binding.


Source

Fusion protein consisting of domains from H. sapiens, S. cerivisiae, e.coli, herpes simplex virus.

References